Rate constants for actin polymerization in ATP determined using cross-linked actin trimers as nuclei.
نویسندگان
چکیده
منابع مشابه
Rate constants for the reactions of ATP- and ADP-actin with the ends of actin filaments
I measured the rate of elongation at the barbed and pointed ends of actin filaments by electron microscopy with Limulus sperm acrosomal processes as nuclei. With improvements in the mechanics of the assay, it was possible to measure growth rates from 0.05 to 280 s-1. At 22 degrees C in 1 mM MgCl2, 10 mM imidazole (pH 7), 0.2 mM ATP with 1 mM EGTA or 50 microM CaCl2 or with EGTA and 50 mM KCl, t...
متن کاملThe interaction between ATP-actin and ADP-actin. A tentative model for actin polymerization.
The involvement of interactions between ATP-actin and ADP-actin in actin polymerization has been studied. It has been found that ATP-actin and ADP-actin can copolymerize and that the rate of nucleation is enhanced when both ATP-actin and ADP-actin are present in solution. The fact that the heterologous interaction between ATP-actin (T) and ADP-actin (D) is stronger than either of the homologous...
متن کاملPolymerization of ADP-actin and ATP-actin under sonication and characteristics of the ATP-actin equilibrium polymer.
Polymerization under sonication has been developed as a new method to study the rapid polymerization of actin with a large number of elongating sites. The theory proposed assumes that filaments under sonication are maintained at a constant length by the constant input of energy. The data obtained for the reversible polymerization of ADP-actin under sonication have been successfully analyzed acc...
متن کاملDirect measurement of actin polymerization rate constants by electron microscopy of actin filaments nucleated by isolated microvillus cores
We used actin filament bundles isolated from intestinal brush-border microvilli to nucleate the polymerization of pure muscle actin monomers into filaments. Growth rates were determined by electron microscopy by measuring the change in the length of the filaments as a function of time. The linear dependence of the growth rates on the actin monomer concentration provided the rate constants for m...
متن کاملProbing actin polymerization by intermolecular cross-linking
We have used N,N'-1,4-phenylenebismaleimide, a bifunctional sulfhydryl cross-linking reagent, to probe the oligomeric state of actin during the early stages of its polymerization into filaments. We document that one of the first steps in the polymerization of globular monomeric actin (G-actin) under a wide variety of ionic conditions is the dimerization of a significant fraction of the G-actin ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1984
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)47223-1